Purified Anti-Human SSEA-4 Antibody[MC-813-70] (AN015520P)
| Alternate Names | Stage-Specific Embryonic Antigen-4, SSEA4 |
| Clone | |
| Verified Samples | Verified Samples in FCM: NCCIT |
| Dilution | FCM ≤ 0.2 µg per million cells in 100 µL volume |
| Background | The MC813-70 antibody reacts with a carbohydrate antigen which is named as Stage-Specific Embryonic Antigen-4 (SSEA-4). It is a terminal structure of globo-series glycolipids (NeuAcα2-3Galβ1-3GalNAcβ1-R). SSEA-4 is expressed on human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos, subset of mesenchymal stem cells, erythrocytes, and erythromyeloid leukemia cell line K562. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-4 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos. |
| Immunogen | Recombinant Human SSEA-4 protein |
| Abbre | SSEA-4 |
| Host | Mouse |
| Reactivity | Human |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Mouse IgG3, κ |
| Applications |
FCM
|
| Research Areas | Cell Biology;Immunology;Neuroscience;Neuroscience Cell Markers;Stem Cells |
| Cellular Localization |
Membrane
|
| Concentration |
≥1 mg/mL
|
| Conjugation | Unconjugated |
| Storage Buffer | Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
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