Purified Anti-Mouse CD14 Antibody[Sa14-2] (E-AB-F1176A)
For research use only.
Conjugation: Unconjugated
APC Elab Fluor®488 Elab Fluor®647 Elab Fluor®Red 780 Elab Fluor®Violet 450 FITC PE PE/Cyanine 5 PE/Cyanine 5.5 PE/Cyanine 7 PerCP/Cyanine 5.5 Unconjugated Show More
| Alternate Names | CD 14, CD14 Antigen, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, Cd14, LPS R, Mo2 |
| Clone | |
| Leadtime | Inquire |
| Verified Samples | Verified Samples in FCM: Mouse peritoneal macrophages |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells) |
| Background | CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed on macrophages, dendritic cells, Kupffer cells, hepatocytes, and granulocytes. As a high-affinity receptor for LPS-LBP (LPS-binding protein) complex, CD14, in association with Toll-like Receptor 4 (TLR4) or 2 (TLR2), is involved in the clearance of gram-negative pathogens. |
| Immunogen | Recombinant Mouse CD14 protein |
| Abbre | CD14 |
| Swissprot | |
| Host | Rat |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Rat IgG2a, κ |
| Isotype Control | Purified Rat IgG2a, κ Isotype Control[2A3] |
| Applications |
FCM
|
| Cellular Localization |
Cell membrane
|
| Tissue Specificity |
Detected on peritoneal macrophages (at protein level). Cell surface expression detected in lung alveolar macrophages, dendritic macrophages and lung macrophages (at protein level).
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation | Unconjugated |
| Storage Buffer | Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
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