Purified Anti-Mouse CD144 Antibody[BV13], Functional Grade (AN010330)
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For research use only.
| Alternate Names | CDH, Cadherin 5 Type, Cadherin, 7B4, AA408225, Cadherin 5 Type 2, Vec, VEcad, VE-Cad, VECD, Cadherin-5, Cdh5, VE-cadherin, CD144, Vascular endothelial cadherin, Cadherin-5 |
| Clone | |
| Verified Samples | Verified Samples in FCM: BEND.3 cells |
| Dilution | FCM ≤ 0.2 µg per million cells in 100 µL volume;Block Reported in the literature |
| Background | The cadherin (Ca++-dependent adherence) superfamily is a large group of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least five major subfamilies based on molecule gene structure, and/or extracellular (EC) and intracellular domains . Subfamilies include classical/type I, atypical/type II, and desmosomal-related cadherins. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Mouse CD144 protein |
| Abbre | CD144 |
| Swissprot | |
| Host | Rat |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Rat IgG1, κ |
| Isotype Control | Purified Rat IgG1, κ Isotype Control[HRPN], Functional Grade |
| Applications |
FCM;Block
|
| Research Areas | Invasion and Metastasis;Cadherins;Cell Adhesion Molecules;Tight Junctions;Cytoskeleton;CDs;Endothelial Cells |
| Cellular Localization |
Cell junction, Cell membrane, Cytoplasm, Membrane
|
| Tissue Specificity |
Expressed in postnatal endothelial cells of the retinal vascular plexus (at protein level).
|
| Concentration |
≥ 3 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Corada M, et al. Proc Natl Acad Sci USA. 1999 Aug 17;96(17):9815-9820. |
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