Purified Anti-Mouse CD3ε Antibody[145-2C11], Functional Grade (E-AB-F11030)
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For research use only.
| Alternate Names | CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E |
| Clone | |
| Leadtime | Order now, ship in 3 days |
| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);Activ Reported in the literaturee;Depletion Reported in the literature;Stim Reported in the literature |
| Background | CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Mouse CD3ε protein |
| Abbre | CD3ε |
| Swissprot | |
| Host | Armenian Hamster |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Armenian Hamster IgG |
| Isotype Control | Purified Armenian Hamster IgG Isotype Control[PIP], Functional Grade |
| Applications |
FCM;Activ;Depletion;Stim
|
| Research Areas | Immunology |
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Ariella Glasner, et al. Immunity. 2018 Jan 16;48(1):107-119.e4.Noga Ron-Harel, et al. Cell Metab. 2016 Jul 12;24(1):104-17. |
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