Purified Anti-Mouse CD45RO/B220 Antibody[RA3-6B2] (AN004280P)
For research use only.
Conjugation: Unconjugated
APC Elab Fluor®488 Elab Fluor®647 Elab Fluor®Violet 450 FITC PE PE/Cyanine 5 PE/Cyanine 7 PE/Elab Fluor®594 PerCP/Cyanine 5.5 Unconjugated Show More
| Clone | |
| Leadtime | Inquire |
| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
| Dilution | FCM ≤ 0.2 µg per million cells in 100 µL volume |
| Background | CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity. |
| Immunogen | Recombinant Mouse B220 protein |
| Abbre | CD45RO |
| Host | Rat |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Rat IgG2a, κ |
| Isotype Control | Purified Rat IgG2a, κ Isotype Control[2A3] |
| Applications |
FCM
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation | Unconjugated |
| Storage Buffer | Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
Note: Data for this module come from published literature that cites this product, displaying other products used together in the same research paper.
Searching publications using CD45RO as the keyword shows that the gene above is closely associated with CD45RO in research. Results are sorted in descending order of research relevance. (The displayed numbers represent the number of publications in which both terms co-occur.)
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