Purified Anti-Mouse IL-6 Antibody[MP5-20F3], Functional Grade (E-AB-F12070)
![Purified Anti-Mouse IL-6 Antibody[MP5-20F3], Functional Grade - 1](http://file.elabscience.com/assets/images/loading.png)
For research use only.
Conjugation: None (AF/LE)
APC Elab Fluor®700 None (AF/LE) PE Unconjugated Show More
| Verified Samples | Verified Samples in FCM: HEK293T cells transfected with pcDNA3.1 plasmid encoding Mouse IL-6 gene |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells), Neut Reported in the literature |
| Isotype | Rat IgG1, κ |
| Host | Rat |
| Reactivity | Mouse |
| Applications | FCM, Neut |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human IL-6 protein |
| Isotype Control | Purified Rat IgG1, κ Isotype Control[HRPN], Functional Grade |
| Abbre | IL-6 |
| Synonyms | BSF, Interleukin, Interleukin HP, IFNB, B-cell hybridoma growth factor, BSF2, CDF, HGF, HSF, IFNB2, IL 6, Interleukin HP-1, Interleukin-6, Il-6, Il6 |
| Swissprot | |
| Cellular Localization | Secreted |
| Concentration | ≥ 1 mg/mL |
| Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Research Areas | Immunology, Innate Immunity |
| Clone | MP5-20F3 |
| Conjugation | None (AF/LE) |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Shipping | Ice bag |
| background | Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature mouse IL-6 is 187 amino acids (aa) in length that is typically expressed as a 22-28 kDA molecular weight protein. Mouse IL-6 shares 39% and 85% aa sequence identity with human and rat IL-6, respectively. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
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