Purified Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114], Functional Grade (E-AB-F09900)
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For research use only.
| Alternate Names | H2-Ab1/Eb1, I-E beta MHC class II, MHC class II, MHC class II H2-IA-beta-psi, Major histocompatibility protein class II beta chain |
| Clone | |
| Leadtime | Order now, ship in 3 days |
| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);Block Reported in the literaturee;FA Reported in the literature |
| Background | These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Mouse MHC class II protein |
| Abbre | MHC II (I-A/I-E) |
| Swissprot | |
| Host | Rat |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Rat IgG2b, κ |
| Isotype Control | Purified Rat IgG2b, κ Isotype Control[LTF-2], Functional Grade |
| Applications |
FCM;Block;FA
|
| Research Areas | Immunology;Innate Immunity |
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Sebastian Kreiter, et al. Nature. 2015 Apr 30;520(7549):692-6.Hongmei Fu, et al. Nat Commun. 2014 Mar 14:5:3436. |
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