Purified Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114], Functional Grade (E-AB-F09900)
![Purified Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114], Functional Grade - 1](http://file.elabscience.com/assets/images/loading.png)
For research use only.
Conjugation: None (AF/LE)
APC Biotin Elab Bright™Violet 421 Elab Bright™Violet 510 Elab Fluor®488 Elab Fluor®647 Elab Fluor®700 Elab Fluor®Violet 450 Elab Fluor®Violet 500 Elab Fluor®Violet 540 Elab Fluor®Violet 610 FITC None (AF/LE) PE PE/Cyanine 5 PE/Cyanine 5.5 PE/Cyanine 7 PerCP PerCP/Cyanine 5.5 Unconjugated Show More
| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells), Block Block - Reported in the literature, FA FA - Reported in the literature |
| Isotype | Rat IgG2b, κ |
| Host | Rat |
| Reactivity | Mouse |
| Applications | FCM, Block, FA |
| Clonality | Monoclonal |
| Immunogen | Recombinant Mouse MHC class II protein |
| Abbre | MHC II (I-A/I-E) |
| Synonyms | H2-Ab1/Eb1, I-E beta MHC class II, MHC class II, MHC class II H2-IA-beta-psi, Major histocompatibility protein class II beta chain |
| Swissprot | |
| Concentration | ≥ 1 mg/mL |
| Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Purification Method | >98%, Protein A/G purified |
| Research Areas | Immunology, Innate Immunity |
| Clone | M5/114 |
| Conjugation | None (AF/LE) |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Shipping | Ice bag |
| background | These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Cat.No. | Product Name | Clone No. |
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