Purified Anti-Mouse TNF-α Antibody[TN3-19.12], Functional Grade (AN008310)
![Purified Anti-Mouse TNF-α Antibody[TN3-19.12], Functional Grade - 1](http://file.elabscience.com/assets/images/loading.png)
| Verified Samples | Verified Samples in FCM: HEK293T cells transfected with pcDNA3.1 plasmid encoding Mouse TNF-α gene |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells), Neut Reported in the literature |
| Isotype | Armenian Hamster IgG2, κ |
| Host | Armenian Hamster |
| Reactivity | Mouse |
| Applications | FCM, Neut |
| Clonality | Monoclonal |
| Immunogen | Recombinant Mouse TNF-α protein |
| Abbre | TNF-α |
| Synonyms | Tumor necrosis factor ligand superfamily member, TNFSF, DIF, Tnfa, TNF-a, TNFSF2, Tnlg1f, Tnfsf1a, TNFalpha, TNF-alpha, ICD1, ICD2, Membrane Form, N-terminal fragment, NTF, Tnf, Cachectin, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tnfa, TNF-a, TNFalpha, TNF-alpha, Tnfsf1a, TNFSF2, Tnlg1f, TNFSF2, TNF, TNFa |
| Swissprot | |
| Cellular Localization | Cell membrane, Membrane, Secreted |
| Concentration | ≥ 1 mg/mL |
| Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Purification Method | >98%, Protein A/G purified |
| Clone | TN3-19.12 |
| Conjugation | None (AF/LE) |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Shipping | Ice bag |
| background | Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Mouse TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
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