Purified Anti-Rat CD3 Antibody[G4.18], Functional Grade (E-AB-F12280)
![Purified Anti-Rat CD3 Antibody[G4.18], Functional Grade - 1](http://file.elabscience.com/assets/images/loading.png)
For research use only.
Conjugation: None (AF/LE)
APC Elab Fluor®700 Elab Fluor®Red 780 Elab Fluor®Violet 540 FITC None (AF/LE) PE PE/Elab Fluor®594 PerCP/Cyanine 5.5 Unconjugated Show More
| Verified Samples | Verified Samples in FCM: Rat splenocytes |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells), FA FA - Reported in the literature, Activ Activ - Reported in the literature |
| Isotype | Mouse IgG3, κ |
| Host | Mouse |
| Reactivity | Rat |
| Applications | FCM, FA, Activ |
| Clonality | Monoclonal |
| Immunogen | Recombinant Rat CD3 protein |
| Abbre | CD3 |
| Synonyms | Cd3g |
| Swissprot | |
| Cellular Localization | Membrane |
| Concentration | ≥ 1 mg/mL |
| Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Purification Method | >98%, Protein A/G purified |
| Clone | G4.18 |
| Conjugation | None (AF/LE) |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Shipping | Ice bag |
| background | CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
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