QuicKey Pro Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit (E-OSEL-H0063)
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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 0.09 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Volume | 50 μL |
| Manual Operation Time | 30 min |
| Total Assay Time | 1 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes NT-proBNP in samples.No significant cross-reactivity or interference between NT-proBNP and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NT-proBNP . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NT-proBNP , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 13 nm. The OD value is proportional to the concentration of NT-proBNP . You can calculate the concentration of NT-proBNP in the samples by comparing the OD of the samples to the standard curve.
Background
May affect cardio-renal homeostasis.Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32.
| Gene Alias | NPPB |
| Gene ID | 4879 |
| Uniport ID | P16860 |
| Protein Alias | NPPB |
| Research Area | Cardiovascular , Neuroscience |
| Cat.No. | Product Name | Clone No. |
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