Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	1.13 ng/mL
Detection Range	3.13-200 ng/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reactivity	Monkey
Specificity	This kit recognizes Monkey Cortisol in samples.No significant cross-reactivity or interference between Monkey Cortisol and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Monkey Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Monkey Cortisol are added to the micro ELISA plate wells. Monkey Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Monkey Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Cortisol is the primary glucocorticoid hormone released by the body in response to stress, with chronic cortisol levels leading to various pathophysiologies.This hormone is regulated by the hypothalamic–pituitary–adrenal (HPA) axis, a complex neuroendocrinological system . Most cortisol in blood (90%) is bound to a carrier protein, cortisol binding globulin (CBG), with free cortisol only making up 5–10% of the total cortisol in circulation.Only free cortisol is biologically active, with bound cortisol being physiologically inactive. Cortisol controls a wide range of physiological processes, such as promoting gluconeogenesis, reducing inflammation, suppressing the immune system and modulating cognitive processes.These responses anticipate and assist the body’s adaptation to stressful conditions by providing energy for awakening, fuelling a “fight or flight” response, and diverting resources to deal with a stressor.

Research Area

Cell Biology , Metabolism