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QuicKey Pro Rat PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA Kit (E-OSEL-R0016)

QuicKey Pro Rat PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA Kit - 1
  • QuicKey Pro Rat PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA Kit - 1
  • QuicKey Pro Rat PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA Kit - 2
  • QuicKey Pro Rat PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA Kit - 3
All Size Price Qty
96T $ 520.00
- +
48T $ 416.00
- +
24T $ 150.00
- +
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity 0.47 ng/mL
Detection Range 0.78-50 ng/mL
Sample Volume 50 μL
Manual Operation Time 30 min
Total Assay Time 1 h 30 min
Reacitivity Rat
Specificity This kit recognizes PCSK9 in samples.No significant cross-reactivity or interference between PCSK9 and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 6 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PCSK9 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PCSK9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PCSK9 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 24 nm. The OD value is proportional to the concentration of PCSK9 . You can calculate the concentration of PCSK9 in the samples by comparing the OD of the samples to the standard curve.
Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na+ channel (ENaC)-mediated Na+ absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
Gene Alias Pcsk9
Gene ID 298296
Uniport ID P59996
Protein Alias Pcsk9
Research Area Signal Transduction , Metabolism , Neuroscience
QuicKey Pro Rat PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA Kit - procedures
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