Rat Hepatic Stellate Cells Isolation and Culture Kit (P-CA-612)
For research use only.
| Product Introduction | The Rat Hepatic Stellate Cells Isolation and Culture Kit is specially developed for extracting primary Rat Hepatic Stellate Cells. As validated, standard operation using this kit enables the acquisition of one flask of cells (T-25 culture flask) per 1 Test,with a cell count exceeding 1 × 10⁶ cells. The cells can be passaged at a ratio of 1:2 for about 3 times. Through immunofluorescence identification, the cell purity (α - SMA positive rate) has been confirmed to exceed 90%. |
| Expiration Date | 12 months |
| Matters Need Attention |
1. Prior to formal experiments, it is recommended to conduct anatomical simulation training using 1-2 normal rats to familiarize operators with procedural workflows and improve tissue dissociation efficiency. 2. During the entire separation process, it is recommended to place the culture dish containing the tissue on an ice plate (2-8℃) to maintain low temperature, but be careful not to freeze the tissue and liquid due to low temperature, so as not to damage the tissue and affect the extraction efficiency. 3. Reagent preparation or dispensing must strictly adhere to aseptic technique protocols. After dispensing, seal the containers immediately with a sealing film, use them promptly to avoid repeated freeze-thaw cycles orcontamination. |
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