Rat JAG1 (Jagged 1 Protein) ELISA Kit (E-EL-R3067)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat JAG1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat JAG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat JAG1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat JAG1 . You can calculate the concentration of Rat JAG1 in the samples by comparing the OD of the samples to the standard curve.

Rat Jagged 1 was the first ligand for Notch identified in mammals. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for activating Notch signaling. Jagged 1 is synthesized as a precursor protein that contains a 21 aa signal sequence, a 1048 aa extracellular region, a 25 aa transmembrane (TM) segment and a short, 226 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats, and a cysteine-rich (CR) region . The extracellular region of rJagged 1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors. Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature. For instance soluble, non-transmembrane forms of Jagged 1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis. Soluble Jagged 1 is also capable of maintaining the survival and enhancing the expansion of stem cells that are capable of reconstituting hematopoietic lineages in vivo. Furthermore, Jagged 1 is implicated in disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged 1. Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling. Rat Jagged 1 shows 98% and 99% aa identity to and Rat Jagged 1 extracellular domains respectively. Relative to the extracellular region of rat Jagged 2, the aa identity is 58%.