Rat Kupffer Cell Isolation and Culture Kit (P-CA-613)
For research use only.
| Product Introduction | This kit is developed specifically for extracting primary rat kupffer cells. After low-temperature digestion with mixed enzymes and density gradient separation, the cells were purified using differential adhesion method to obtain rat kupffer cells. Laboratory validation showed that 1 × 10⁶ target cells could be isolated in each experiment (1 Test). Immunofluorescence identification showed that the cell purity (CD68 positive rate) was > 90%. |
| Endotoxin Level (EU/mL) | < 3 |
| Shipping | Ice bag |
| Expiration Date | 12 months |
| Matters Need Attention |
1. The experiment requires the preparation of dissection plates, ice plate, 0.25% trypsin digestion solution, and surgical instruments (including 2 ophthalmic scissors, 2 surgical straight forceps, and 1 surgical curved forceps); tissue processing dishes (glass culture dishes, 6 cm/10 cm are acceptable), 6 cm culture dishes, and several 15 mL/50 mL centrifuge tubes. 2. Before the formal experiment, it is recommended to use 1-2 normal rats for simulated anatomy to familiarize the operation process and improve the speed of tissue separation. 3. During the entire separation process, it is recommended to place the culture dish containing the tissue on an ice plate (2-8℃) to maintain low temperature, but be careful not to freeze the tissue and liquid. 4. The culture medium contains nutrients necessary for microbial growth. Please open it in an ultra-clean workbench, divide it according to the required amount, and seal the bottle mouth with a sealing film for immediate use to avoid contamination. 5. All separation solutions in this kit may experience the precipitation of crystal particles at the bottle mouth and turbidity of the solution during storage, which is a normal phenomenon and can be used with confidence. |
| System Certification |
GMP Production and Quality System The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's Rat Kupffer Cell Isolation and Culture Kit complies with ISO 9001. |
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® 430
Elab Fluor® 488
Elab Fluor® 568
Elab Fluor® 594
Elab Fluor® 647
Elab Fluor® 680
Elab Fluor® 700
Elab Fluor® 750
Elab Fluor® Red 780
Elab Fluor® Violet 450
Elab Fluor®488
Elab Fluor®594
Elab Fluor®647
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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