Rat PGF (Placental Growth Factor) CLIA Kit (E-CL-R0520)

Name: Rat PGF (Placental Growth Factor) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0520
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat PGF (Placental Growth Factor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	1.88 pg/mL
Detection Range	3.13-200 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat PGF in samples.No significant cross-reactivity or interference between Rat PGF and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
200	42672.000	48232.000	45452.000	45420
100	17079.000	19401.000	18240.000	18208
50	8723.000	8131.000	8427.000	8395
25	3965.000	4569.000	4267.000	4235
12.5	2028.000	1960.000	1994.000	1962
6.25	1132.000	1018.000	1075.000	1043
3.13	583.000	659.000	621.000	589
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	9.380	23.950	97.830	8.910	22.410	100.360
Standard deviation	0.970	2.160	7.870	0.870	2.200	8.680
CV（%）	10.340	9.020	8.040	9.760	9.820	8.650

Recovery

The recovery of Rat PGF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-111	104
EDTA plasma （n=8）	85-98	91
Cell culture media （n=8）	97-111	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat PGF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	102-114	85-99	90-105
1:2	Average	109	91	98
1:4	Range	85-95	101-114	94-105
1:4	Average	90	106	99
1:8	Range	103-117	91-107	101-120
1:8	Average	108	97	110

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PGF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PGF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat PGF. You can calculate the concentration of Rat PGF in the samples by comparing the RLU value of the samples to the standard curve.

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