Recombinant 14-3-3 theta Monoclonal Antibody (AN301741L)
For research use only.
| Verified Samples | Verified Samples in WB: IMR-32, Caco-2, Mouse kidney, Rat testis |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human 14-3-3 theta fragment |
| Abbre | 14-3-3 theta |
| Synonyms | 14-3, YWHAQ, 3-Mar, HS1, 14-3-3, 1C5, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14 3 3 tau, 14 3 3 theta, 14-3-3 protein tau, 14-3-3 protein T-cell, 14-3-3 protein theta, 1433T, 1C5, KCIP1, Protein HS1, Protein kinase C inhibitor protein 1, Protein tau, theta, theta polypeptide, tyr3/trp5 monooxygenase activation protein, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein |
| Swissprot | |
| Calculated MW | 28 kDa |
| Observed MW |
28 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Signal Transduction, Stem Cells |
| Clone No. | A449 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | 14-3-3 protein theta is a protein that in humans is encoded by the YWHAQ gene. This gene product belongs to the 14-3-3 family of proteins that mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence. |
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Unconjugated
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