Recombinant 5T4 Monoclonal Antibody (AN301765L)
For research use only.
| Verified Samples |
Verified Samples in WB: MCF-7, JAR Verified Samples in IHC: Human placenta Verified Samples in IP: MCF-7 cells extracts |
| Dilution | WB 1:500-1:1000, IHC 1:1000-1:2000, IP 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human 5T4 fragment |
| Abbre | 5T4 |
| Synonyms | M6P, WAIF, TPBG, 5T4, 5T4AG, M6P1, WAIF1, 5T4 Protein, 5T4AG Protein, Human, M6P1 Protein, WAIF1 Protein |
| Swissprot | |
| Calculated MW | 46 kDa |
| Observed MW |
70-80/85 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Stem Cells, Cancer, Developmental Biology |
| Clone No. | A473 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Trophoblast glycoprotein (encoded by 5T4) is a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. This protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. |
Other Clones
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Other Formats
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Unconjugated
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