Recombinant ACE2 Monoclonal Antibody (AN300933L)
For research use only.
| Verified Samples | Verified Samples in WB: CACO-2 |
| Dilution | WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human ACE2 protein |
| Abbre | ACE2 |
| Synonyms | ACE, UNQ, PRO, Metalloprotease MPROT, Angiotensin-converting enzyme, ACE2, ACEH, ACEII, Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, ACE-related carboxypeptidase, Metalloprotease MPROT15, Processed angioten, UNQ868, PRO1885, ACEH, angiotensin-converting enzyme 2, ACE 2, ACE related carboxypeptidase, Angiotensin converting enzyme 2, Angiotensin converting enzyme homolog, Angiotensin converting enzyme like protein, Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2, Angiotensin I converting enzyme 2, DKFZP434A014, EC 3.4.17, metalloprotease MPROT 15, OTTHUMP00000022963, Processed angiotensin-converting enzyme 2 |
| Swissprot | |
| Calculated MW | 130 kDa |
| Observed MW |
130 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Cardiovascular |
| Clone No. | 7A5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. |
Other Clones
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Unconjugated
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