Recombinant ACTH Monoclonal Antibody (AN301965L)
For research use only.
| Dilution | WB 1:1000, IHC-P 1:500-1:2000, ICC/IF 1:100 |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P, ICC/IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant protein of human POMC |
| Abbre | ACTH |
| Synonyms | POMC, ACTH, CLIP, LPH, MSH, NPP, POC, Adrenocorticotropin, alpha-intermedin, alpha-melanocortin, alpha-melanotropin, b-EP, Pro-opiomelanocortin, Corticotropin-lipotropin, Gamma-MSH, Corticotropin, Alpha-MSH, Beta-LPH, Gamma-LPH, Beta-MSH, Beta-endorphin, Met-enkephalin, Melanotropin gamma, Potential peptide, Adrenocorticotropic hormone, Alpha melanocyte stimulating hormone, alphaMSH, Beta LPH, Beta melanocyte stimulating hormone, Corticotropin lipotropin, Corticotropin-like intermediary peptide, Gamma LPH, included, Lipotropin, Lipotropin beta, Lipotropin gamma, Melanocyte-stimulating hormone, Melanotropin, Melanotropin alpha, Melanotropin beta, Opiomelanocortin prepropeptide, Pomc1, Pomc-1, Pomc2, Pro ACTH endorphin, Pro opiomelanocortin, Proopiomelanocortin, Proopiomelanocortin preproprotein, Pro-opiomelanocortin-alpha, Tetracosactide, γ-intermedin, γ-melanocortin, γ-melanotropin, alpha-MSH, beta-MSH, gamma-MSH |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW |
29 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Signal Transduction, Cancer, Metabolism |
| Clone | C111586 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | ACTH, adrenocorticotropic hormone, is a peptide hormone cleavage product of the POMC protein produced by the pituitary gland. ACTH plays an important role in stress and circadian rhythm signaling. Its primary action is on the adrenal cortex, where its binding induces the secretion of glucocorticoid steroid hormones. The peptide sequence for this hormone is highly evolutionarily conserved amongst mammals. Specifically, for mouse and rat, this sequence shares 100% identity. |
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