Recombinant Adiponectin Monoclonal Antibody (AN300978L)
For research use only.
| Verified Samples | Verified Samples in WB: Rat fat |
| Dilution | WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Adiponectin protein |
| Abbre | Adiponectin |
| Synonyms | GBP, ACRP, ADIPQTL, APM, ADIPOQ, ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28, Adiponectin, Adipocyte, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein (ACRP30), C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein, ACDC, ACRP30, ADIPOQ, APM1, GBP28, ACRP30-HMW, ACRP30-HMW, apM-1-HMW, ACDC-HMW, Adipocyte complement-related 30 kDa protein, APM1-HMW, GBP28-HMW, Adiponectin(ADP/Acrp30) |
| Swissprot | |
| Calculated MW | 26 kDa |
| Observed MW |
30 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Tissue Specificity | Adipocyte |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Signal Transduction, Neuroscience, Stem Cells, Cancer, Metabolism |
| Clone No. | 11A1 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | ADIPOQ(adiponectin, C1Q and collagen domain containing) Homo sapiens This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
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Unconjugated
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