Recombinant ALDOA Monoclonal Antibody (AN301431L)
For research use only.
| Verified Samples |
Verified Samples in WB: MCF7, HepG2, Mouse kidney, Rat brain Verified Samples in IHC: Human colon cancer Verified Samples in IF: Jurkat |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human ALDOA fragment |
| Abbre | ALDOA |
| Synonyms | GSD, Lung cancer antigen NY-LU, Fructose, ALDOA, ALDA, GSD12, HEL-S-87p, 6 Bisphosphate Aldolase, Fructose 1, Muscle-Type Aldolase, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, 6-BISPHOSPHATE ALDOLASE A, Aldo1, Aldolase, Aldolase 1, Aldolase A, Aldolase A fructose bisphosphatase, Aldolase A fructose bisphosphate, Epididymis secretory sperm binding protein Li 87p, FRUCTOALDOLASE A, Fructose 1 6 bisphosphate triosephosphate lyase, Fructose bisphosphate aldolase, Fructose bisphosphate aldolase A, FRUCTOSE-1, fructose-bisphosphate A, Fructose-bisphosphate aldolase A Muscle-type, HEL S 87p, Lung cancer antigen NY LU 1, MGC10942, MGC17716, MGC17767, Muscle type aldolase, RNALDOG5 |
| Swissprot | |
| Calculated MW | 39 kDa |
| Observed MW |
35 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cancer, Metabolism |
| Clone No. | A126 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein. |
Other Clones
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Other Formats
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Unconjugated
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