Recombinant ALKBH2 Monoclonal Antibody (AN301228L)
For research use only.
| Verified Samples | Verified Samples in WB: HeLa |
| Dilution | WB 1:1000-1:5000, |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human ALKBH2 protein |
| Abbre | ALKBH2 |
| Synonyms | ABH, ALKBH, ALKBH2, ABH2, 2OG Fe(II) oxy DC1, 9530023G02, ABH 2, alkB alkylation repair homolog 2, ALKB2, ALKBH2 alkB, Alkylated DNA repair protein alkB homolog, Alkylated DNA repair protein alkB homolog 2, Alkylation repair homolog 2, Alpha ketoglutarate dependent dioxygenase alkB homolog 2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, AU016977, DNA oxidative demethylase ALKBH2, EC 1.14.11., FLJ99103, hABH2, mABH2, MGC90512, Oxy DC1 |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW |
29 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, Nucleus, nucleolus, Nucleus, nucleoplasm, Relocates to the replication foci during S-phase. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | 4B15 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]). |
Other Clones
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Unconjugated
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