Recombinant alpha smooth muscle Actin Monoclonal Antibody (AN301435L)

Name: Recombinant alpha smooth muscle Actin Monoclonal Antibody
Brand: Elabscience
SKU: AN301435L
Price: 249.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

Recombinant alpha smooth muscle Actin Monoclonal Antibody - 1

All	Size	Price	Qty
	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Verified Samples	Verified Samples in WB: MCF-7, HeLa, A431, HEK-293, NIH-3T3, Rat colon, Mouse colon Verified Samples in IHC: Human prostate hyperplasia, Mouse stomach, Rat stomach Verified Samples in IF: HeLa, C6 Verified Samples in FCM: HeLa, Jurkat Verified Samples in IP: HeLa cells extracts
Dilution	WB 1:500-1:2000, IHC 1:1000-1:5000, IF 1:50, FCM 1:50-1:100, IP 1:50-1:100
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, Rat, Mouse
Applications	WB, IHC, IF, FCM, IP
Clonality	Monoclonal;Recombinant
Immunogen	Recombinant human alpha smooth muscle Actin fragment
Abbre	alpha smooth muscle Actin
Synonyms	AAT, GIG, MYMY, Growth inhibiting gene, AAT6, ACTSA, MYMY5, ACTA2, actin, a actin, Actin aortic smooth muscle, Growth inhibiting gene 46, ACTVS, GIG46, Actin alpha 2 smooth muscle aorta, Alpha 2 actin, Alpha actin 2, Alpha cardiac actin, alpha sma, Alpha-actin-2, aortic smooth muscle, Cell growth inhibiting gene 46 protein, Cell growth-inhibiting gene 46 protein, α-SMA, a actin, AAT6, ACTA, ACTA2, Actin aortic smooth muscle, Actin, ACTSA, ACTVS, GIG46, Growth inhibiting gene 46, MYMY5
Swissprot	P62736
Calculated MW	42 kDa
Observed MW	42 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Cytoplasm, cytoskeleton
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Signal Transduction, Stem Cells, Cardiovascular, Developmental Biology
Clone No.	A130
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	Alpha smooth muscle actin encoded by the ACTA2 gene is a key protein in the highly conserved actin family, which plays a crucial role in cell motility, structure and integrity. This isoform is predominantly expressed in vascular smooth muscle cells, where it is essential for maintaining the structure and function of blood vessels and other smooth muscle tissues. Mutations in ACTA2 are linked to various vascular disorders, including familial thoracic aortic aneurysms and dissections (TAAD) and multisystemic smooth muscle dysfunction syndrome.