Recombinant ATG3 Monoclonal Antibody (AN300723L)
For research use only.
| Verified Samples |
Verified Samples in WB: 3T3-L1 Verified Samples in IHC: Human esophageal cancer |
| Dilution | WB 1:500-1:3000, IHC 1:200-1:1000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human ATG3 protein |
| Abbre | ATG3 |
| Synonyms | APG, PC3, ATG, Protein PC3, Autophagy-related protein, Ubiquitin-like-conjugating enzyme ATG, hApg, ATG3, APG3, APG3-LIKE, APG3L, PC3-96, Autophagy-related protein 3, hApg3, Protein PC3-96, Ubiquitin-like-conjugating enzyme ATG3, 2610016C12Rik, Apg 3, APG3 autophagy 3 like, APG3 like, Apg3p, ATG 3, ATG3 autophagy related 3 homolog, ATG3 autophagy related 3 homolog (S. cerevisiae), Autophagy 3, Autophagy Apg3p/Aut1p like, autophagy related 3, Autophagy related protein 3, DKFZp564M1178, FLJ22125, homolog of, MGC15201, OTTHUMP00000214547, OTTHUMP00000214548, PC3 96, S. cerevisiae |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW |
36 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Neuroscience, Cancer, Cardiovascular, Metabolism |
| Clone No. | 11A5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. |
Other Clones
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Unconjugated
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