Recombinant ATG5 Monoclonal Antibody (AN300864L)
For research use only.
| Verified Samples |
Verified Samples in WB: U-87 MG Verified Samples in IHC: Human colon tissue |
| Dilution | IHC 1:200-1:1000, WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human ATG5 protein |
| Abbre | ATG5 |
| Synonyms | ATG, APG, hAPG, Autophagy protein, ATG5, APG5, APG5-LIKE, APG5L, ASP, hAPG5, Apoptosis-specific protein, Autophagy protein 5, APG 5, APG 5L, APG5 autophagy 5 like, APG5 like, Apoptosis specific protein, ATG 5, ATG5 autophagy related 5 homolog, Autophagy related 5, Homolog of S Cerevisiae autophagy 5, OTTHUMP00000040507 |
| Swissprot | |
| Calculated MW | 33 kDa |
| Observed MW |
55 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Cardiovascular, Cancer, Metabolism |
| Clone No. | 8B5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. |
Other Clones
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Other Formats
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Unconjugated
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