Recombinant ATG9A Monoclonal Antibody (AN301795L)

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For research use only.
Verified Samples |
Verified Samples in WB: HepG2, HeLa Verified Samples in IHC: Human thyroid cancer Verified Samples in IF: HepG2 Verified Samples in FCM: HepG2 |
Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50, FCM 1:50-1:100 |
Isotype | IgG, κ |
Host | Rabbit |
Reactivity | Human, |
Applications | WB, IHC, IF, FCM |
Clonality | Monoclonal;Recombinant |
Immunogen | Recombinant human ATG9A fragment |
Abbre | ATG9A |
Synonyms | MGD, mATG, APG9L, ATG9A, APG9L1, MGD3208, mATG9, APG9 autophagy 9-like 1, APG9 like 1, APG9-like 1, ATG9, ATG9 autophagy related 9 homolog A, ATG9 autophagy related 9 homolog A (S. cerevisiae), Autophagy 9-like 1 protein, Autophagy related 9A, Autophagy related protein 9A, Autophagy-related protein 9A, OTTHUMP00000206046, OTTHUMP00000206048, OTTHUMP00000206049, OTTHUMP00000206062 |
Swissprot | |
Calculated MW | 94 kDa |
Observed MW |
100 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Endoplasmic reticulum, Golgi apparatus, Endosome |
Concentration | 1 mg/mL |
Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
Purification Method | Protein A purified |
Research Areas | Cell Biology, Neuroscience, Cancer, Cardiovascular, Metabolism |
Clone No. | A507 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | ATG9A are lipid scramblases that play a role in autophagosome expansion. ATG9A is present in numerous intracellular compartments, including the trans-Golgi network and early and recycling endosomes, and traffics through the secretory pathway to the plasma membrane and to the endocytic pathway from the plasma membrane. ATG9A protects cells against plasma membrane damage caused by a spectrum of exogenous and endogenous agents, including permeabilization by gasdermin and mixed lineage kinase domain like (MLKL), which generate pores at the plasma membrane or perturb plasma membrane integrity, respectively, during programmed cell death processes of pyroptosis and necroptosis. We furthermore define the ATG9A–IQGAP1 apparatus that integrates with the ESCRT system to cooperatively heal areas of plasma membrane damage. |
Other Clones
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