Recombinant BAF60C Monoclonal Antibody (AN301723L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, K-562, C6 Verified Samples in IHC: Human endometrial cancer, Mouse kidney Verified Samples in IF: HeLa |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human BAF60C fragment |
| Abbre | BAF60C |
| Synonyms | CRACD, SMARCD, Rsc6p, BAF60C, CRACD3, SMARCD3, BAF60C, CRACD3 |
| Swissprot | |
| Calculated MW | 55 kDa |
| Observed MW |
70 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Epigenetics and Nuclear Signaling, Cardiovascular, Cancer, Metabolism |
| Clone No. | A431 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | BAF60C, also known as SMARCD3, is a protein that is encoded by the SMARCD3 gene in human. BAF60C is a member of the SWI/SNF family of proteins, it displays helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. BAF60C is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. BAF60C together with TBX15 triggers development glycolytic fast-twitch muscles by the activation of the Akt/PKB signaling pathway. |
Other Clones
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Other Formats
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Unconjugated
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