Recombinant Bcl-XL Monoclonal Antibody (AN301019L)
For research use only.
| Verified Samples |
Verified Samples in WB: 3T3-L1 Verified Samples in IHC: Human colon carcinoma tissue |
| Dilution | IHC 1:200-1:1000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Bcl-XL protein |
| Abbre | Bcl-XL |
| Synonyms | Bcl-2-Like Protein, PPP1R, Bcl2-L, BCL2 like, BCL2L1, BCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52, BCL2 like 1, Bcl-XL, Apoptosis Regulator Bcl-X, Bcl2-L-1, Bcl-2-Like Protein 1, BCLXL, BCLXS, bcl-xS, Apoptosis regulator Bcl X, Apoptosis regulator BclX, B cell lymphoma 2 like, B2CL1, Bcl 2 like 1 protein, Bcl X, Bcl xL, BCL XL/S, Bcl xS, Bcl2 related gene, DKFZp781P2092, Protein phosphatase 1 regulatory subunit 52, Bcl-x |
| Swissprot | |
| Calculated MW | 26 kDa |
| Observed MW |
30 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Signal Transduction, Cancer, Metabolism |
| Clone No. | 3P6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. |
Other Clones
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Unconjugated
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