Recombinant BMP-2 Monoclonal Antibody (AN300061P)
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For research use only.
| Verified Samples |
Verified Samples in WB: Hela, MCF7, RAW264.7, Rat spleen in IHC: Rat spine in IF: HEK293, HEK293 |
| Dilution | IHC 1:200-1:1000, WB 1:1000-1:5000, IF 1:200-1:1000, ELISA 1:5000-1:20000, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human / Mouse / Rat / Rhesus / Canine BMP-2 protein |
| Abbre | BMP2 |
| Synonyms | BDA, BMP, BDA2, BMP2A, BMP2, BDA2A, BMP-2, BMP-2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A |
| Swissprot | |
| Calculated MW | 45kD,13kD |
| Observed MW |
30kD,13kD
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Stem Cells, Cancer, Cardiovascular |
| Clone | A1171 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016] |
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