Recombinant BMP2 Monoclonal Antibody (AN302121L)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela, MCF7, RAW264.7, Rat spleen Verified Samples in IHC: Rat spine Verified Samples in IF: HEK293 |
| Dilution | IHC 1:200-1:1000, WB 1:1000-1:5000, IF 1:200-1:1000, ELISA 1:5000-1:2000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | IHC, WB, IF, ELISA |
| Immunogen | Recombinant protein corresponding to Mouse BMP2 |
| Abbre | BMP2 |
| Synonyms | BDA, BMP, BDA2, BMP2A, BMP2, BDA2A, BMP-2, BMP-2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A, Bmp-2, Bmp2, BDA, BDA2, BMP2A, BMP-2A, Bmp-2, Bmp2 |
| Swissprot | |
| Calculated MW | 45, 13 kDa |
| Observed MW |
30, 13 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Stem Cells, Cancer, Cardiovascular |
| Clone No. | A0362R |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Bone morphogenetic protein-2(BMP-2) is a menmber of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer, 2 monomersthen associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-teminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TCF beta signaling pathway and cytokine-cytokine receptorinteraction. |
| Cat.No. | Product Name | Clone No. |
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