Recombinant BNI3L Monoclonal Antibody (AN301123L)
For research use only.
| Verified Samples | Verified Samples in WB: MCF7 |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human BNI3L protein |
| Abbre | BNI3L |
| Synonyms | BNIP3L, BNIP3a, NIX, BNIP3H, Adenovirus E1B19k binding protein B5, Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kd protein interacting protein 3a, BCL2/adenovirus E1B 19 kDa protein interacting protein 3A, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, BCL2/adenovirus E1B 19kDa interacting protein 3 like, BNI3L, BNIP3L protein, NIP3 like protein X, NIP3L, NIP3-like protein X, BNI3L |
| Swissprot | |
| Calculated MW | 24 kDa |
| Observed MW |
38 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus envelope. Endoplasmic reticulum. Mitochondrion outer membrane. Membrane, Single-pass membrane protein, Colocalizes with SPATA18 at the mitochondrion outer membrane. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Cancer, Cardiovascular, Metabolism |
| Clone No. | 12G6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | BCL2 interacting protein 3 like(BNIP3L) Homo sapiens This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c. |
Other Clones
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Unconjugated
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