Recombinant c-Maf Monoclonal Antibody (AN301761L)
For research use only.
| Verified Samples | Verified Samples in WB: Rat brain, Mouse brain |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human c-Maf fragment |
| Abbre | c-Maf |
| Synonyms | CCA, CTRCT, MAF, AYGRP, CCA4, CTRCT21, c-MAF, Proto-oncogene c-Maf, Transcription factor Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog, AS42 oncogene homolog, Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene, Avian musculoaponeurotic fibrosarcoma (v maf), c maf proto oncogene, cMaf, MAF2, MGC71685, Proto oncogene c Maf, v maf musculoaponeurotic fibrosarcoma oncogene homolog, v maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) |
| Swissprot | |
| Calculated MW | 38 kDa |
| Observed MW |
45 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Immunology, Cancer |
| Clone No. | A469 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Transcription factor Maf (encoded by MAF) is a DNA-binding, leucine zipper-containing transcription factor that acts as a homodimer or as a heterodimer. Depending on the binding site and binding partner, the encoded protein can be a transcriptional activator or repressor. This protein plays a role in the regulation of several cellular processes, including embryonic lens fiber cell development, increased T-cell susceptibility to apoptosis, and chondrocyte terminal differentiation. |
Other Clones
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Unconjugated
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