Recombinant Casein Kinase 2 beta Monoclonal Antibody (AN301820L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, MCF-7, NIH/3T3, C6 Verified Samples in IHC: Human colon cancer, Rat cerebrum Verified Samples in IF: NIH/3T3 |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Casein Kinase 2 beta fragment |
| Abbre | Casein Kinase 2 beta |
| Synonyms | Ckb, CK2B, CK2N, CSK2B, G5A, CSNK2B, Ckb1, Ckb2, Casein kinase 2 beta polypeptide, Casein kinase II beta subunit, Casein kinase II subunit beta, CK II beta, CSNK 2B, MGC138222, MGC138224, Phosvitin, Protein G5a, casein kinase 2 beta |
| Swissprot | |
| Calculated MW | 25 kDa |
| Observed MW |
25 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus, secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Stem Cells |
| Clone No. | A532 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Casein Kinase 2 beta is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit. |
Other Clones
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Other Formats
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Unconjugated
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