Recombinant CD117/c-Kit Monoclonal Antibody (AN301303L)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse brain |
| Dilution | WB 1:500-1:2000, |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human CD117/c-Kit protein |
| Abbre | CD117 |
| Synonyms | KIT, C-Kit, CD117, PBT, SCFR, Mast/stem cell growth factor receptor Kit, p145 c-kit, Piebald trait protein, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, SCFR, C Kit, CD_antigen: CD117, c-Kit Ligand, Kit Ligand, KIT oncogene, KIT proto oncogene receptor tyrosine kinase, KIT proto-oncogene receptor tyrosine kinase, Mast cell growth factor receptor, MGF, Proto oncogene c Kit, Proto oncogene tyrosine protein kinase Kit, SCF Receptor, soluble KIT variant 1, Steel Factor Receptor, Stem cell factor receptor, tyrosine protein kinase Kit, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein, c Kit/CD117, c-Kit |
| Swissprot | |
| Calculated MW | 110 kDa |
| Observed MW |
130 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasmic, Membranous |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Immunology, Tags & Cell Markers, Signal Transduction, Neuroscience, Stem Cells, Cancer, Developmental Biology |
| Clone No. | 7C3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. |
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Unconjugated
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