Recombinant CD13 Monoclonal Antibody (AN301461L)
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For research use only.
| Verified Samples |
Verified Samples in WB: THP-1 Verified Samples in IHC: Human breast, Human kidney, Mouse kidney, Rat kidney Verified Samples in IF: THP-1, A375 Verified Samples in FCM: THP-1 |
| Dilution | WB 1:500-1:2000, IHC 1:200-1:1000, IF 1:50-1:100, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CD13 fragment |
| Abbre | CD13 |
| Synonyms | LAP, ANPEP, APN, CD13, GP150, LAP1, P150, PEPN, Aminopeptidase N, AP-M, AP-N, CD13 antigen, CD13APN, PEPNhAPN, gp150, Alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, AMPN, AP M, AP N, CD 13, hAPN, LAP 1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13 |
| Swissprot | |
| Calculated MW | 110 kDa |
| Observed MW |
150 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Stem Cells |
| Clone No. | A156 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity. |
Other Clones
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Other Formats
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Unconjugated
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