Recombinant CD133 Monoclonal Antibody (AN301089L)
For research use only.
| Verified Samples |
Verified Samples in WB: Caco-2 Verified Samples in IHC: Human bladder carcinoma tissue |
| Dilution | IHC 1:20-100, WB 1:1000-5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human CD133 protein |
| Abbre | CD133 |
| Synonyms | PROM, CORD, MCDR, MSTP, STGD, PROML, PROM1, AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4, Prominin-1, Prominin-1, AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4, Antigen AC133, Hematopoietic stem cell antigen, hProminin, OTTHUMP00000217744, OTTHUMP00000217745, OTTHUMP00000217746, Prominin I, Prominin like 1, Prominin like protein 1 precursor, Prominin mouse like 1, Prominin1, Prominin-like protein 1 |
| Swissprot | |
| Calculated MW | 97 kDa |
| Observed MW |
133 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membranous |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Stem Cells |
| Clone No. | 6G13 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Other Formats
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Unconjugated
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