Recombinant CD147/EMMPRIN/Basigin Monoclonal Antibody (AN300025P)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, Ramos in IHC: Human colon cancer in IF: HeLa |
| Dilution | WB: 1:1000-1:5000；IHC-P: 1:200-1:500；ICC/IF: 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P, ICC/IF |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human CD147 protein |
| Abbre | BSG |
| Synonyms | UNQ, Membrane glycoprotein gp, BSG, 5F7, CD147, EMMPRIN, OK, TCSF, basigin, Basic immunoglobulin superfamily, basigin (Ok blood group), Collagenase stimulatory factor, EMPRIN, Extracellular matrix metalloproteinase inducer, HT7 antigen, Leukocyte activation antigen M6, M6, Membrane glycoprotein gp42, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, UNQ6505, PRO21383, Neurothelin, basigin, EMMPRIN, 5A11 antigen, BASI, Blood brain barrier HT7 antigen, CD 147, CD147 antigen, M 6, M6 leukocyte activation antigen, OK blood group, Tumor cell derived collagenase stimulatory factor |
| Swissprot | |
| Calculated MW | 42 kDa |
| Observed MW |
50 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Neuroscience, Cancer, Metabolism, Cardiovascular |
| Clone | A1135 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
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