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Recombinant CD147/EMMPRIN/Basigin Monoclonal Antibody (AN300025P)

All Size Price Qty
100μL $ 380.00
20μL $ 150.00
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For research use only.

Verified Samples Verified Samples in WB: A431, Hela
Verified Samples in FCM: HeLa
Verified Samples in IHC: Human stomach, Human rectal cancer, Human placenta, Human liver, Human gastric cancer, Human esophagus
Dilution WB 1:500-1:2000,  FCM 1:25-1:100,  IHC-P 1:2500-1:10000,  IHC-Fr 1:500-1:2000,  ICC/IF 1:50-1:1000,  
Isotype IgG
Host Rabbit
Reactivity Human
Applications WB,  IHC-P,  IHC-Fr,  FCM,  ICC/IF
Clonality Rabbit Monoclonal
Immunogen Recombinant Human CD147 protein
Abbre BSG
Synonyms UNQ,  Membrane glycoprotein gp,  BSG,  5F7,  CD147,  EMMPRIN,  OK,  TCSF,  basigin,  Basic immunoglobulin superfamily,  basigin (Ok blood group),  Collagenase stimulatory factor,  EMPRIN,  Extracellular matrix metalloproteinase inducer,  HT7 antigen,  Leukocyte activation antigen M6,  M6,  Membrane glycoprotein gp42,  OK blood group antigen,  Tumor cell-derived collagenase stimulatory factor,  UNQ6505,  PRO21383,  Neurothelin,  basigin,  EMMPRIN,  5A11 antigen,  BASI,  Blood brain barrier HT7 antigen,  CD 147,  CD147 antigen,  M 6,  M6 leukocyte activation antigen,  OK blood group,  Tumor cell derived collagenase stimulatory factor
Swissprot
Calculated MW 42 kDa
Observed MW 35-55 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cell membrane
Concentration 1 mg/mL
Buffer 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0
Purification Method Protein A
Research Areas Immunology,  Neuroscience,  Cancer,  Metabolism,  Cardiovascular
Clone No. 6C11
Conjugation Unconjugated
Storage This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Shipping Ice bag
background The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene.
Other Clones

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Unconjugated

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