Recombinant CD22 Monoclonal Antibody (AN300016P)
For research use only.
| Verified Samples |
Verified Samples in WB: Raji, K562, Ramos in IF: HL-60 |
| Dilution | WB: 1:1000；IHC-P: 1:200-1:2000；ICC/IF: 1:100；IP: 1:20-1:50 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, ICC/IF, IP |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human CD22 protein |
| Abbre | CD22 |
| Synonyms | Lyb, SIGLEC2FLJ, B-cell receptor CD, T-cell surface antigen Leu, sialic acid binding Ig-like lectin, Sialic acid-binding Ig-like lectin, CD22, SIGLEC-2, SIGLEC2, B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule, CD22 antigenMGC130020, CD22 molecule, Lyb-8, sialic acid binding Ig-like lectin 2, Sialic acid-binding Ig-like lectin 2, SIGLEC2FLJ22814, T-cell surface antigen Leu-14, Siglec-2, CD22 molecule, B cell receptor CD22 precursor, B lymphocyte cell adhesion molecule, BL CAM, BLCAM, CD 22, CD22 antigen, CD22 protein, Lectin 2, Leu14, Lyb8, MGC130020, sialic acid binding Ig like lectin 2, Sialic acid binding immunoglobulin like lectin 2, SIGLEC 2, T cell surface antigen Leu 14 |
| Swissprot | |
| Calculated MW | 95 kDa |
| Observed MW |
140 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Cancer, Stem Cells |
| Clone | A1126 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. |
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