Recombinant CD27/TNFRSF7 Monoclonal Antibody (AN300006P)
For research use only.
| Verified Samples | Verified Samples in WB: Raji |
| Dilution | WB 1:500-1:2000, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human CD27/TNFRSF7 Protein |
| Abbre | CD27 |
| Synonyms | S152. LPFS, T-cell activation antigen CD, TNFRSF, S152.LPFS, CD27, S152, S152. LPFS2, T14, TNFRSF7, Tp55, S152.LPFS2, CD27L receptor, T-cell activation antigen CD27, S152, TNFRSF7, CD 27, CD27 antigen, CD27 molecule, LPFS2, MGC20393, OTTHUMP00000238557, T cell activation antigen CD27, T cell antivation antigen S152, TNFRSF 7, TNFSF7, Tp 55, Tumor necrosis factor receptor superfamily member 7 |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW |
65 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Cancer, Stem Cells |
| Clone No. | 2B8 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. |
Other Clones
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Other Formats
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Unconjugated
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