Recombinant CD31 Monoclonal Antibody (AN300911L)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2 Verified Samples in IHC: Mouse skin |
| Dilution | IHC 1:1000-1:2000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human CD31 protein |
| Abbre | CD31 |
| Synonyms | PECA, cluster of differentiation, PECAM1, CD31, CD31/EndoCAM, GPIIA, PECA1, PECAM-1, endoCAM, cluster of differentiation 31, Platelet endothelial cell adhesion molecule, endocam, Adhesion molecule, CD31 antigen, CD31 EndoCAM, FLJ34100, FLJ58394, GPIIA', PECAM, Pecam 1, PECAM 1 CD31 EndoCAM, PECAM1, Platelet and endothelial cell adhesion molecule 1, Platelet/endothelial cell adhesion molecule 1, CD31, EndoCAM, GPIIA, PECA1, PECAM-1, Platelet endothelial cell adhesion molecule |
| Swissprot | |
| Calculated MW | 81 kDa |
| Observed MW |
130 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Signal Transduction, Stem Cells, Cancer |
| Clone No. | 8C12 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | PECAM1(platelet and endothelial cell adhesion molecule 1) Homo sapiens The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. |
Other Clones
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Other Formats
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Unconjugated
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