Recombinant CD31 Monoclonal Antibody (E-AB-81462)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat Verified Samples in IHC: Human lung cancer |
| Dilution | WB 1:1000-1:5000, IHC 1:20-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human CD31 |
| Abbre | CD31 |
| Synonyms | Adhesion molecule, CD31, CD31 EndoCAM, CD31 antigen, EndoCAM, FLJ34100, FLJ58394, GPIIA, GPIIA', PECA1, PECAM, PECAM 1 CD31 EndoCAM, PECAM-1, Pecam 1, Pecam1, Platelet and endothelial cell adhesion molecule 1, Platelet endothelial cell adhesion molecule, Platelet/en |
| Swissprot | |
| Calculated MW | 83 kDa |
| Observed MW |
130 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Cardiovascular, Immunology, Signal Transduction, Stem Cells |
| Clone No. | R07-6A1 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Platelet endothelial cell adhesion molecule-1 (PECAM-1,CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. It is highly expressed on the surface of the endothelium,making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets,monocytes,neutrophils,natural killer cells,megakaryocytes and some types of T-cell . As well as its role in cell-cell adhesion,PECAM-1 functions as a signaling receptor,and is involved in important physiological events such as nitric oxide production,regulation of T-cell immunity and tolerance,leukocyte transendothelial migration and inflammation and angiogenesis . |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
