Recombinant CD3D Monoclonal Antibody (AN301813L)

Name: Recombinant CD3D Monoclonal Antibody
Brand: Elabscience
SKU: AN301813L
Price: 249.0 USD
Availability: InStock

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Recombinant CD3D Monoclonal Antibody - 1

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	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

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Verified Samples	Verified Samples in WB: THP-1(Negative control), Jurkat, HuT-78, MOLT-4 Verified Samples in IHC: Human tonsil Verified Samples in IF: Jurkat Verified Samples in FCM: Jurkat, THP-1(Negative cell line) Verified Samples in IP: Jurkat cells extracts
Dilution	WB 1:2000-1:10000, IHC 1:200-1:1000, IF 1:50-1:200, FCM 1:50-1:100, IP 1:25-1:50
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human,
Applications	WB, IHC, IF, FCM, IP
Clonality	Monoclonal;Recombinant
Immunogen	Recombinant human CD3D fragment
Abbre	CD3D
Synonyms	IMD, CD3D, CD3-DELTA, IMD19, T3D, T-Cell Receptor T3 Delta Chain, T-cell surface glycoprotein CD3 delta chain, T3, CD3 delta
Swissprot	P04234
Calculated MW	19 kDa
Observed MW	23 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Membrane
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Immunology, Stem Cells
Clone No.	A525
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	CD3D is a component of the TCR-CD3 complex, which is present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition, CD3D plays an essential role in thymocyte differentiation. It participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. It interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.