Recombinant CD40 Monoclonal Antibody (AN301709L)
For research use only.
| Verified Samples |
Verified Samples in WB: Raji Verified Samples in IHC: Human Hodgkin lymphoma |
| Dilution | WB 1:1000-1:5000, IHC 1:200-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CD40 fragment |
| Abbre | CD40 |
| Synonyms | CDW, B-cell surface antigen CD, Tumor necrosis factor receptor superfamily member, TNFRSF, CD40, Bp50, CDW40, TNFRSF5, p50, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, CD40L receptor, TNFRSF5, AI326936, B cell associated molecule CD40, B cell surface antigen CD40, B cell-associated molecule, CD 40, CD40 (protein), CD40 antigen, CD40 antigen (TNF receptor superfamily member 5), CD40 molecule, CD40 protein, CD40 type II isoform, GP39, HIGM1, IGM, IMD3, isoform CRA_a, member 5, MGC9013, Nerve growth factor receptor related B lymphocyte activation molecule, OTTHUMP00000031699, OTTHUMP00000031700, TBAM, T-BAM, TNF receptor superfamily member 5, TNR5, TRAP, Tumor necrosis factor receptor superfamily, Tumor necrosis factor receptor superfamily member 5 precursor |
| Swissprot | |
| Calculated MW | 32 kDa |
| Observed MW |
44-48 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane and secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Cardiovascular, Stem Cells |
| Clone No. | A417 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The CD40 antigen is a 44-48 kDa type I integral membrane protein of the tumor necrosis factor receptor (TNFR) superfamily. This antigen is found on B cell lines, is strongly expressed by interdigitating cells (IDC), basal epithelial cells, and is also present on macrophages, some endothelial cells, and follicular dendritic cells. It is a well-known regulator of T cell immunity including in cancer. CD40-ligand, primarily expressed on activated T cells, interacts with CD40 on APCs resulting in a ‘licensed’ state. |
Other Clones
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Other Formats
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Unconjugated
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