Recombinant CD47 Monoclonal Antibody (AN301936L)
For research use only.
| Verified Samples | Verified Samples in WB: HepG2, Jurkat, 293T, A549, U-2 OS, Molt4, HL-60 |
| Dilution | WB 1:1000-1:2000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CD47 fragment |
| Abbre | CD47 |
| Synonyms | MER, Protein MER, Leukocyte surface antigen CD, Antigenic surface determinant protein OA, CD47, IAP, MER6, OA3, Integrin-associated protein, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein (IAP), Protein MER6, Antigen identified by monoclonal 1D8, CD 47, CD47 antigen, CD47 antigen (Rh-related antigen, CD47 glycoprotein, CD47 molecule, Integrin Associated Protein, Integrin associated signal transducer, integrin-associated signal transducer), MER 6, OA 3, OTTHUMP00000041152, OTTHUMP00000041153, Rh related antigen, Surface antigen identified by monoclonal 1D8 |
| Swissprot | |
| Calculated MW | 47-52 kDa |
| Observed MW |
47-52 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Cancer |
| Clone No. | A652 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. CD47 plays an important role in memory formation and synaptic plasticity in the hippocampus. CD47 is a receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 interacts with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. CD47 may prevent premature elimination of red blood cells. CD47 may be involved in membrane permeability changes induced following virus infection. |
Other Clones
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Other Formats
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Unconjugated
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