Recombinant CD48 Monoclonal Antibody (AN301698L)
For research use only.
| Verified Samples |
Verified Samples in WB: Raji Verified Samples in IHC: Human colon Verified Samples in IF: Raji |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:100, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CD48 fragment |
| Abbre | CD48 |
| Synonyms | MEM, hCD, mCD, BCM, sgp, TCT, Leukocyte antigen MEM, B-lymphocyte activation marker BLAST, HM48, SLAMF, CD48, BCM1, BLAST, BLAST1, MEM-102, SLAMF2, hCD48, mCD48, BCM1 surface antigen, BLAST-1, B-lymphocyte activation marker BLAST-1, CD48 antigen, HM48-1, Leukocyte antigen MEM-102, MRC OX-45 surface antigen, sgp-60, TCT.1, BCM1, Sgp-60, SLAMF2, Antigen CD48, B cell membrane protein, B lymphocyte activation marker BLAST 1, B-cell activation marker, BCM 1 surface antigen, BLAST 1, CD 48, CD48 antigen (B cell membrane protein), CD48 molecule, CD48 protein, Leucocyte antigen MEM 102, MEM 102, MEM102, Signaling lymphocytic activation molecule 2, SLAM family member 2, SLAMF 2 |
| Swissprot | |
| Calculated MW | 28 kDa |
| Observed MW |
40 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology |
| Clone No. | A406 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation. |
Other Clones
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Other Formats
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Unconjugated
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