Recombinant CD63 Monoclonal Antibody (AN300839L)
For research use only.
| Verified Samples | Verified Samples in WB: NIH-3T3 |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human CD63 protein |
| Abbre | CD63 |
| Synonyms | MLA, LAMP, Tetraspanin, CD antigen CD, Melanoma-associated antigen ME, Lysosomal-associated membrane protein, CD63, LAMP-3, ME491, MLA1, OMA81H, TSPAN30, CD63 antigen, CD antigen CD63, CD63 molecule, Granulophysin, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, LIMP, MLA1, LAMP-3, CD 63, CD63 antigen (melanoma 1 antigen), CD63 antigen melanoma 1 antigen, gp55, LAMP 3, LAMP3, Lysosomal associated membrane protein 3, Lysosome associated membrane glycoprotein 3, Mast cell antigen AD1, Melanoma 1 antigen, Melanoma associated antigen ME491, Melanoma associated antigen MLA1, MGC72893, MLA 1, NGA, Ocular melanoma associated antigen, PTLGP40, Tetraspanin 30, Tspan 30 |
| Swissprot | |
| Calculated MW | 26 kDa |
| Observed MW |
30-65 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Immunology, Tags & Cell Markers, Cancer |
| Clone No. | 2D11 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. |
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Unconjugated
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