Recombinant CD70 Monoclonal Antibody (AN301838L)
For research use only.
| Verified Samples | Verified Samples in WB: SH-SY5Y(Negative control), SK-OV-3 |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CD70 fragment |
| Abbre | CD70 |
| Synonyms | Tumor necrosis factor ligand superfamily member, TNFSF, CD70, CD27-L, CD27L, CD27LG, TNFSF7, TNLG8A, CD27 ligand, CD70 antigen, Tumor necrosis factor ligand superfamily member 7, Ki-24, CD27L, TNFSF7, CD 27L, CD 70, CD27 L, CD27 LG, CD70 molecule, Ki 24 antigen, Ki24 antigen, member 7, sCD27 Ligand, soluble CD27 Ligand, Surface antigen CD70, TNFSF 7, Tumor necrosis factor (ligand) superfamily |
| Swissprot | |
| Calculated MW | 23 kDa |
| Observed MW |
27,54,81 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Cancer, Stem Cells |
| Clone No. | A550 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | CD70 is a type II transmembrane glycoprotein and a member of the tumor necrosis factor ligand superfamily (TNFSF), also known as CD27L and TNFSF7. It is normally expressed on medullary thymic epithelial cells. Its expression is induced on activated lymphoid cells (B cells, T cells, and NK cells) and dendritic cells. CD70 is a ligand for CD27, a co-stimulatory receptor that plays an important role in T cell activation and proliferation. CD70 overexpression has been reported in various tumors such as renal cell carcinoma, glioblastoma, and non-small cell lung carcinoma and it’s being actively pursued as a therapeutic target. |
Other Clones
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Other Formats
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Unconjugated
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