Recombinant CD74 Monoclonal Antibody (AN301823L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat (Negative control), Raji, Ramos Verified Samples in IHC: Human spleen, Human tonsil Verified Samples in FCM: Ramos, Jurkat(Negative cell line) |
| Dilution | WB 1:500-1:2000, IHC 1:2000-1:5000, FCM 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human CD74 fragment |
| Abbre | CD74 |
| Synonyms | HLA class II histocompatibility antigen gamma chain, HLADG, Ia antigen-associated invariant chain, Ia-GAMMA II, Ii, p33, CD74 HLA-DR antigens-associated invariant chain, DHLAG, CD74, invariant chain, CD 74, CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, CD74 molecule, class II antigen-associated), class II invariant chain, CLIP, Gamma chain of class II antigens, HG2A, HLA DR antigens associated invariant chain, HLA DR gamma, HLADR antigens associated invariant chain, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, Ia antigen associated invariant chain, Ia associated invariant chain, Ia gamma, Ia-GAMMA, Invariant polypeptide of major histocompatibility complex class II antigen associated, la-gamma, major histocompatibility complex, Major histocompatibility complex class II invariant chain, MHC HLA DR gamma chain, MHC HLA-DR gamma chain, p35, Protein 41, CD74, DHLAG, HLA class II histocompatibility antigen gamma chain, HLADG, Ia antigen-associated invariant chain, p33, CD 14, CD_antigen=CD14, CD14, CD14 antigen, CD14 molecule, LPS-R, Mo2, Monocyte differentiation antigen CD14, Monocyte differentiation antigen CD14 urinary form, membrane-bound form, Myeloid cell specific leucine rich glycoprotein, Myeloid cell-specific leucine-rich glycoprotein |
| Swissprot | |
| Calculated MW | 34 kDa |
| Observed MW |
34 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology |
| Clone No. | A535 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | CD74, also known as the MHC Class II-associated invariant chain (Ii), is a type II transmembrane glycoprotein that plays a critical role in the antigen presentation process as a chaperone of MHC Class II proteins. It is expressed at high levels on B cells and to a lesser extent on numerous antigen presenting cell (APC) types including dendritic cells, Langerhans cells, monocytes, and macrophages as well as non-traditional APCs such as epithelial cells. CD74 was initially identified for its ability to regulate the folding and intracellular trafficking of newly synthesized MHC Class II molecules. Following expression, CD74 self-assembles as a trimer that serves as a scaffold for the assembly of MHC Class II molecules. Through this interaction, CD74 blocks the peptide binding cleft of MHC Class II molecules and prevents their premature association with endogenous polypeptides. Binding to CD74 also facilitates the translocation of MHC Class II molecules from the endoplasmic reticulum to the endocytic compartments during antigen presentation. |
Other Clones
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