Recombinant CD84/SLAMF5 Monoclonal Antibody (AN300093P)
For research use only.
| Dilution | WB: 1:1000-1:5000;IHC-P: 1:100-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human CD84 protein |
| Abbre | CD84 |
| Synonyms | hCD, mCD, SLAM family member, Cell surface antigen MAX, Leukocyte differentiation antigen CD, Signaling lymphocytic activation molecule, SLAMF, Cell surface antigen MAX.3, hCD84, Hly9-beta, Leukocyte differentiation antigen CD84, LY9B, mCD84, Signaling lymphocytic activation molecule 5, SLAM family member 5, SLAMF5, CD84, GR6 |
| Swissprot | |
| Calculated MW | 39 kDa |
| Observed MW |
45-80 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Stem Cells |
| Clone | A1207 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011] |
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